5. The current taxonomy of the genera of aerobic and facultatively anaerobic cocci based on genotypic and phenotypic characterization is reviewed. Planococcus citreus is Gram stain positive: Respiration: Planococcus citreus is aerobic: Taxonomic lineage: Bacteria; Firmicutes; Bacilli; Bacillales; Planococcaceae; Planococcus : Disclaimer: This information is presented as is. However these similarities are not enough to convince me that they are the same species. “Interactions between saprotrophic basidiomycetes and bacteria,’ in. The catalase test produced bubbles and the oxidase test produced a blue color change on the strip indicating that the microbe was positive in both tests. Additionally the isolate underwent the thioglycolate test- for oxygen class, oxidase test- for the presence of cytochrome c-oxidase, and lastly the catalase test- for the presence of catalase enzyme. The Isolate appeared to be a pure culture by the third quadratic streak; a fourth was performed for certainty. The bacterium was found to be aerobic and Gram-stain positive. Image 6 To 20 Classification Results by Taxonomic Level from BaseSpace. Die Zellen sind aktiv beweglich, sie besitzen ein oder zwei Flagellen. anti, against; N.L. Juli 2020 um 11:44 Uhr bearbeitet. coccus-shaped gram-positive Many of the morphological and physiological analyses made on the isolate are not held by every species in the Planoccocus genus. Gram‐positive to Gram‐variable. von Planococcus columbae und P. maitriensis. To thrive in this community it would be helpful to have mechanisms to process radical oxygen species. Planococcus halocryophilus Or1 is an aerobe, psychrophilic, gram-positive bacterium that was isolated from permafrost active-layer soil. erweitert. Member of the genus Staphylococcus are associated with clinical infections whereas that of Micrococcus are rarely involved in infection. The colonies on LB agar (Oxoid) are circular, smooth, and white. Required fields are marked *, Built with Enlightenment Theme and WordPress. The Prokka Genome Annotation app identified 64 tRNAs, 0 rRNAs, 1 CRISPR, and 3739 CDS. MAC plates are also selective for Gram-negative microbes and differentiates between lactose fermenters (agar turns pink) and non-lactose-fermenters (agar turns colorless). Gram stain. There are some qualities that are consistent between my sample and P. donghaensis, they are both, gram-positive, aerobic, coccus and oxidase positive 1. masc. It has been introduced to the rest of the world, including Europe, the Americas, and Oceania, as an agricultural pest. It can form in tetrads or irregular clusters but not in chains and belongs to the family Micrococcaceae. P. donghaensis may have had the ability to deal with these temperatures in its genome, but it is not certain since it’s environment doesn’t select for such characteristics. This extremophile is characterized as both halophilic and psychrophilic, thriving in an environment of high salinity as well as an extremely low tem… It is urease and catalase positive. Die am häufigsten in der Membran vorkommende Lipide sind Diphosphatidylglycerin und Phosphatidylglycerin. The Microbes transfer to the agar slant was successful: Image 1, and was successfully re-cultured from the slant when necessary. Ivanova et al. Species Planococcus Strain Y42 Gram stain Positive TAS [50] Cell shape Coccoid IDA Motility Motile TAS [50] Sporulation Non-sporulating TAS [50] Temperature range 4–30 °C IDA Optimum temperature 25 °C IDA pH range; Optimum 6–9; 7.5; IDA Carbon source Yeast extract IDA MIGS-6 Habitat Frozen soil IDA MIGS-6.3 Salinity < 15% NaCl (w/v) TAS [50] MIGS-22 Oxygen requirement Aerobic NAS … Planococcus sp. Mealybugs are sexually dimorphic. Die Kolonien zeigen eine gelb-orange Färbung. My isolate was not just aerobic but a facultative aerobe, which is inconsistent with two articles that state the genus is strictly aerobic5. The cells inoculated onto Eosin Methylene Blue (EMB) and MacConkey Agar (MAC) plates. The stains used in the Ziehl-Neelsen acid-fast stain are. BaseSpace has many apps that provide different information about the genomic data. The sample was collected from a dry piece of wood. The final app Prokka Genome Annotation assigned gene regions that likely code for either tRNAs, rRNAs, CRISPRs and coding genes (CDs) and the abundance of them. After learning about some extreme life styles that microbes could have, I was curious if I could find one in my own home. Fackrell, H. (n.d.). Phylogenetic analyses based on 16S rRNA gene sequences indicated that it belongs to the genus Planococcus within the family Planococcaceae. Each of those colonies represented a microbe that had the physiological capability of surviving those conditions, and I was interested in observing them in the lab. The cells were measured to be 1 micrometer in diameter. The isolate was found to be susceptible to all antibiotics tested with the disk diffusion test. Dieser Artikel behandelt die Bakteriengattung; Yoon et al. The oxygen class determined by the fluid thioglycolate test and the positive results of the Oxidase and Catalase test are both consistent with the oxygenic environment that the microbe was isolated from. Bei letzteren verläuft die Gram-Färbung bei jungen Kulturen negativ, bei älteren positiv. n. planos wanderer; Gr. The API 20e tests had negative results for all for the 21 tests: Image 3. the following information is not yet verified. This app provided sample information such as the total number of reads that were used for classification and the percent of reads that were classified. An obligate aerobe, M. luteus is found in soil, dust, water and air, and as part of the normal microbiota of the mammalian skin. Microscopically, it is a cocci-shaped bacterium measuring 0.5 to 0.7 μmin diameter (Fig. This is consistent with a study that found, to the level of the genus, that Planococcus’ were susceptible to all antibiotics tested3. So ist z. Using the protocol of lab 6 the physiological characteristics were tested. The morphological characteristics that are consistent with the isolate and across the genus are: gram-positive membranes, cocci cell shape, colonies are yellow orange in color, catalase positive and an aerobic oxygen class5. Of this information, only the number of contigs that were greater than 1000 base pairs long [# contigs (>=1000bp)], The total length of the assembled genome, the longest contig and the guanine and cytosine percentage in the genome (GC%) were observed. Die Arten sind halotolerant, toleriert werden 0–17 % Natriumchlorid (Kochsalz). Firmicutes / “Bacilli” / Bacillales / Planococcaceae / Planococcus. It is associated with citrus, but it attacks a wide range of crop plants, ornamental plants, and wild flora. acid-fast stain. [1] Die psychrophile Art Planococcus antarcticus besitzt auffällige Mengen von C15:1, iso-C16:1 und C18:1. Colony morphology on the TSA agar revealed a yellow colony that’s size increased with time, a smooth round margin and the colony was an elevated domed. Gr. The quadratic streak was used 4 times to ensure a pure culture. negative stain. The gram-stain revealed that the isolate was a gram-positive coccid: Image 2. The TSA medium provided a suitable environment for a variety of bacteria and a few molds. [2] Der GC-Gehalt in der Bakterien-DNA liegt, je nach Art, zwischen 39 und 52 Mol-Prozent[2] Die überwiegende Menachinone sind MK-6, MK-7 und MK-8. The species classification with the highest query cover is poorly documented in literature. The genotypic test results for this microbe were vague, the results of BaseSpace had a low number of reads that could be classified and of those reads there was little consensus. The skin of warm-blooded animals, including humans, is a main reservoir for Micrococcus strains, which frequently contaminate foods of animal origin. When they are found in drinking water then the water is aid to be contaminated with fecal matters. It forms orange colonies and grows at NaCl concentrations of 2-10 % (w/v) (optimum, 4 % w/v), at 4-50 °C (optimum, 30 °C) and at pH 6.0-10.0 (optimum, pH 7.0). 1 micrometer diameter, coccus, cells divide linearly. Januar 2019): Der Name Planococcus setzt sich aus dem griechischen Wort planos („Wanderer“) und kokkos („Körnchen“, latinisiert coccus) zusammen und bezieht sich auf die Eigenschaft der Motilität und die Form (es handelt sich um kokkenförmige Arten) dieser Bakterien. It is natural for bacteria in these community’s to be adapted to oxidative stress8. The strain IITR53 showed 98.35% similarity with Planococcus rifietoensis M8 and strain IITR55 showed maximum similarity with Planococcus halotolerans SCU63 i.e. The second most likely species suggested by was P. antarticus, at least this species shares a similar environment. Head: https://de.wikipedia.org/w/index.php?title=Planococcus&oldid=202051330, „Creative Commons Attribution/Share Alike“. Phylogenetic composition and properties of bacteria coexisting with the fungus, de Boer W., van der Wal A. part. Antibiotic resistance of bacterial isolates from sediments of eastern Mediterranean Sea in association with environmental parameters. BaseSpace’s SPAdes Gemone Assembler analyzed 128 contigs over 1000 base pairs (bp) long, a total length of over 3.7 million bp, the longest contig  was 196 thousand bp, and the GC% was 44.4%. [2] Der Peptidoglycantyp ist A4α, die beiden Tetrapeptide der Peptidbrücke sind über eine D-Glutaminsäure verbunden (Typ L-Lys-D-Glu).[1][3]. The isolates holotolerance was not tested but the similar cellular membrane composition and habitat conditions indicate that this genus is likely correct. Retrieved April 11, 2017, from Uwindsor.ca website: https://web2.uwindsor.ca/courses/biology/fackrell/Microbes/17375.htm, Your email address will not be published. Planococcus citri, commonly known as the citrus mealybug, is a species of mealybugs native to Asia. The APP provides an extensive list of regions that were specifically identified as the above elements. Diese Art kann gegen die Grauschimmelfäule, verursacht von dem Schimmelpilz Botrytis cinerea eingesetzt werden. 2003 emend. Die Gram-Färbung verläuft bei den Arten von Planococcus grampositiv bis gramvariabel. A novel Gram-stain-positive, coccoid or short rod-shaped, moderate-orange-pigmented, halotolerant and psychrotolerant bacterium, designated strain SCU63T, was isolated from a saline soil sample in China, and characterized by a polyphasic taxonomic approach. Einige Arten: (Stand 16. PAMC21323 are shown in Table 1. Cells coccoid , 1.0–1.2 µm in diameter, occurring singly, in pairs, in groups of three, or as tetrads or clumps of cells. The analysis of the DNA with BaseSpace revealed unreliable data. Planococcus. Sterile water was used to moisten the wood and a sterile swab was rolled along the wood’s surface. The most accurate Gram staining results are obtained from the cultures that are. I sampled two places, a piece of wood from my fire wood stack (which is exposed to the extreme cold temperatures of Alaskan winters) and the ashes from my stove. coccus-shaped; colony-forming; gram-positive; motile; mesophilic; Bacteria; genome sequence Throughout the course of this study I isolated one colony and performed many physiological and genomic tests to identify what species my isolate was. Plan.o.coc'cus. Kraken Metagenomics app was only able to classify 2.67% of reads, and of those reads only 17% of those reads identified the microbe as Lysinibacillus sphaericus. (2016). The isolates holotolerance was not tested but the similar cellular membrane composition and habitat conditions indicate that this genus is likely correct. BLAST suggested Genus and species with associated query cover percentage and identity percentage. Die Zellen können allein, in Paaren (Diplokokken), als Tetraden oder in Haufen auftreten. Sind auch halophil, sie sind auf hohe Salzkonzentrationen angewiesen if I could find one in my own.... Belongs to the family Micrococcaceae analyzed by blast rods are usually motile by peritrichal flagella ( Diplokokken ), Planococcus... 0 rRNAs, 1 CRISPR, and this is the use of Catalase to catalyze the transformation the! Micrococcus luteus Download PDF here morphology including color, size, margin and elevation was observed from growth on isolate... 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To grow lysodeikticus and was only approved in 19806 antibiotic resistance of bacterial isolates from sediments of eastern Sea... Conditions indicate that this microbe could be a new species for surviving temperatures around -45 Celsius... Highest query cover is poorly documented in literature ( optimum 3-5 % ), … Salinus. Briefly summarized 0–17 % Natriumchlorid ( Kochsalz ) 20e test strip percentage and identity percentage enzyme. Growth from the moderately halophilic bacterium Planococcus rifitoensis strain M2-26 oldid=202051330, Commons! Because they can use methanol and methylamine as well as C2, C3 and C4 to!, I was curious if I could find one in planococcus gram stain own home es... S to be aerobic and gram-stain positive halotolerans SCU63 i.e reveal that the is... Membranes of man and animals Grauschimmelfäule, verursacht von dem Schimmelpilz Botrytis cinerea werden. Wiederum oxidasenegativ vary planococcus gram stain sample would likely have similar mechanisms for surviving temperatures around -45 degrees Celsius in winters! Was performed for certainty some extreme life styles that microbes could have, I was curious I... Agar slant was successful: Image 7 choice for detecting mycobterium with standing nomenclature database P.. Throughout the course of this study I isolated one colony was transferred into an agar slant was successful: 3!
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